Could you explain a bit more your use-case? Is this question specific to mirrors, or for channels in general?

Assuming the latter:

If permanently adding a channel with a lower priority would work (so that it only gets used if explicitly requested or a package does not exist in prior channels), this does the job for me:

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi workspace channel add https://repo.prefix.dev/bioconda
✔ Added https://repo.prefix.dev/bioconda

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi add https://repo.prefix.dev/bioconda::htslib
✔ Added https://repo.prefix.dev/bioconda::htslib

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi add cowpy
✔ Added cowpy >=1.1.5,<2

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi ls -x
Package  Version  Build         Size      Kind   Source
cowpy    1.1.5    pyhd8ed1ab_2  23.1 KiB  conda  https://conda.anaconda.org/conda-forge/
htslib   1.22.1   hfcd771d_0    2.5 MiB   conda  https://repo.prefix.dev/bioconda/

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ bat pixi.toml
───────┬────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
       │ File: pixi.toml
───────┼────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
   1   │ [workspace]
   2   │ authors = ["Lucas Colley <lucas.colley8@gmail.com>"]
   3   │ channels = ["conda-forge", "https://repo.prefix.dev/bioconda"]
   4   │ name = "temp-channel"
   5   │ platforms = ["osx-arm64"]
   6   │ version = "0.1.0"
   7   │ 
   8   │ [dependencies]
   9   │ htslib = { version = ">=1.22.1,<2", channel = "https://repo.prefix.dev/bioconda" }
  10   │ cowpy = ">=1.1.5,<2"
───────┴────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────

If you do not want the channel to be discoverable at all for future pixi add commands, you can restrict the channel to a feature. E.g.

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi workspace channel add https://repo.prefix.dev/bioconda --feature=my-bioconda-pkgs
✔ Added https://repo.prefix.dev/bioconda

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi workspace environment add default -f my-bioconda-pkgs --force
✔ Updated environment default

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi add -f my-bioconda-pkgs htslib
✔ Added htslib >=1.22.1,<2
Added these only for feature: my-bioconda-pkgs

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi add cowpy
✔ Added cowpy >=1.1.5,<2

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ pixi ls -x
Package  Version  Build         Size      Kind   Source
cowpy    1.1.5    pyhd8ed1ab_2  23.1 KiB  conda  https://conda.anaconda.org/conda-forge/
htslib   1.22.1   hfcd771d_0    2.5 MiB   conda  https://repo.prefix.dev/bioconda/

~/sandbox/temp-channel via 🧚 v0.50.2 
❯ bat pixi.toml
───────┬────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
       │ File: pixi.toml
───────┼────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
   1   │ [workspace]
   2   │ authors = ["Lucas Colley <lucas.colley8@gmail.com>"]
   3   │ channels = ["conda-forge"]
   4   │ name = "temp-channel"
   5   │ platforms = ["osx-arm64"]
   6   │ version = "0.1.0"
   7   │ 
   8   │ [tasks]
   9   │ 
  10   │ [dependencies]
  11   │ cowpy = ">=1.1.5,<2"
  12   │ 
  13   │ [feature.my-bioconda-pkgs]
  14   │ channels = ["https://repo.prefix.dev/bioconda"]
  15   │ 
  16   │ [feature.my-bioconda-pkgs.dependencies]
  17   │ htslib = ">=1.22.1,<2"
  18   │ 
  19   │ [environments]
  20   │ default = ["my-bioconda-pkgs"]
───────┴────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────

docs: add examples for overriding channel priority in pixi add #4231

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docs: add examples for overriding channel priority in `pixi add` #4231